Title of article :
Photo-triggered generation of a free thiol group on DNA: application to DNA conjugation
Author/Authors :
Takada، نويسنده , , Tadao and Kawano، نويسنده , , Yuta and Nakamura، نويسنده , , Mitsunobu and Yamana، نويسنده , , Kazushige، نويسنده ,
Issue Information :
هفته نامه با شماره پیاپی سال 2012
Abstract :
DNA oligomers possessing a 2-nitrobenzyl (NB) protected thiol group have been prepared. The photo-remove of the NB to generate a free thiol group in DNA has been analyzed by using reverse-phase HPLC and denaturing gel electrophoresis. The photo-triggered generation of the thiol function in DNA was applicable in the light-initiated ligation of thiol-modified DNA oligomers and Au–DNA conjugation.
Keywords :
DNA , thiols , Nitrobenzyl group , Photoreaction , DNA conjugation
Journal title :
Tetrahedron Letters
Journal title :
Tetrahedron Letters
