Title of article
β-Cyclodextrin as a mimetic of the natural GFP-chromophore environment
Author/Authors
Cacciarini، نويسنده , , Martina and Nielsen، نويسنده , , Mogens Brّndsted and de Castro، نويسنده , , Elisa Martinez and Marinescu، نويسنده , , Lavinia and Bols، نويسنده , , Mikael، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2012
Pages
4
From page
973
To page
976
Abstract
The green fluorescent protein (GFP) chromophore has been anchored to β-cyclodextrin (βCD) via a copper(I)-catalyzed azide-alkyne cycloaddition. The photophysical properties of this new GFP-CD derivative have been evaluated, showing the formation of a self-inclusion complex and enhancement of fluorescence of the GFP-chromophore covalently bound to the βCD. This enhancement of fluorescence by encapsulation and hence fixation of the chromophore in a binding pocket mimics the effect exerted by the natural protein environment.
Keywords
Inclusion complexes , chromophore , fluorescence , Cyclodextrins , Click reaction , Green Fluorescent Protein (GFP)
Journal title
Tetrahedron Letters
Serial Year
2012
Journal title
Tetrahedron Letters
Record number
1880156
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