Author/Authors :
Garcيa-Beltrلn، نويسنده , , Olimpo and Mena، نويسنده , , Natalia and Berrيos، نويسنده , , Tania A. and Castro، نويسنده , , Enrique A. and Cassels، نويسنده , , Bruce K. and Nٌْez، نويسنده , , Marco T. and Aliaga، نويسنده , , Margarita E.، نويسنده ,
Abstract :
In this Letter we present a new probe, 2-amino-3-hydroxy-2-(hydroxymethyl)propyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (PMR), which can reversibly detect mercuric ions (Hg2+) in HEPES buffer under physiological conditions. Possible interference with other analytes was examined. PMR displays a highly selective decrease of its fluorescence at 460 nm when it reacts with Hg2+. Interestingly, the probe can also be used as a fluorescent turn-on sensor for biologically relevant thiols such as glutathione and cysteine. PMR can be used to determine mercury in living cells.
Keywords :
Fluorescent probe , Hg2+-selectivity , Biothiols , Coumarin derivatives
