Author/Authors :
Fattahian، Yaser نويسنده M.Sc. (Microbiology student), Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeinis shrine, Tehran- 3319118651, Iran Fattahian, Yaser , Rasooli، Iraj نويسنده , , Mousavi Gargari ، Seyed Latif نويسنده Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran , , Sandstr?m، Gunnar نويسنده Karolinska Institutet and Karolinska, University Hospital, Huddinge, Department of Laboratory Medicine, Division of Clinical Microbiology, SE-141 86 Stockholm Sandstr?m, Gunnar , Rahbar، Mohammad Reza نويسنده Molecular Diagnosis Department, Saadati Pathobiology Laboratory, No. 80, Felestin Street, Shiraz-7134668346, Iran Rahbar, Mohammad Reza , Amani، Jafar نويسنده , , Darvish Alipour Astaneh، Shakiba نويسنده 6-Ph.D (Microbiology scholar), Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeinis shrine, Tehran- 3319118651, Iran Darvish Alipour Astaneh, Shakiba
Abstract :
Objective:
Acinetobacter baumannii is an opportunistic pathogen that causes serious infections in humans by
colonization of medical devices. The capacity of this pathogen to persist in hospital settings could be due to its ability to form biofilms. In the present study we evaluated the effect of antibodies against one of surface components of A.baumannii on in vitro biofilm formation.
Materials and Methods:
The 1113 bp fragment of Bap (biofilm associate protein)gene from A. baumannii genome
was amplified and cloned. The recombinant protein was expressed and purified and used to raise antibodies in mice.
Antibody titer was evaluated by ELISA. In vitro biofilm inhibition was evaluated using the mice sera.
Results:
Injection of Bap subunit resulted in high titers of antibody. Immunized mice serum had significant (P <
0.001) inhibitory effect on biofilm formation. In vitro inhibition of biofilm formation by A. baumannii was studied at
1:50 concentrations of immunized and unimmunized mice sera. Immunized mice serum had significant (P < 0.001)
inhibitory effect on biofilm formation
Conclusion:
Adsorption of antibodies to Bap might interfere with bacterial adhesion to the surface. These antibodies
were found to be potent biofilm inhibitors
