Author/Authors :
Muhaghegh-Dolatabady، M. نويسنده Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran , , Habibizad، J. نويسنده Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran , , Bahreini Behzadi، M. R. نويسنده Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran ,
Abstract :
The growth hormone receptor (GHR) is a member of cytokine/hematopoietin family that mediates the biological actions of growth hormone (GH) on target tissues. Therefore, the purpose of this study was to examine the association of TG-repeat polymorphisms in the 5’-flanking region of bovine GHR gene with milk production traits and somatic cell score (SCS) in Holstein cattle of Iran. The part of 5’-flanking region of GHR gene that encompassed TG-repeat was screened by single strand conformation polymorphism (SSCP) method and DNA sequencing. Five hundred eighteen Iranian Holstein cows were genotyped, giving 3 distinct SSCP patterns (A, B, and C). Frequencies of these patterns for the amplified fragment were 0.21, 0.26 and 0.53, respectively. Statistical analysis revealed that TG-repeat had a significant effect on average daily milk production (P < 0.05) and tended to associate with fat and protein percentage. No significant difference was observed between TG-genotypes and SCS. The association identified in the TG-repeat of GHR gene may have potential to serve as candidate genetic marker for marker assisted selection (MAS) in cattle.
