Author/Authors :
Ranjhani، A. نويسنده Sindh Agriculture University Tandojam, India. , , Khatri ، P. نويسنده Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Sindh, Pakistan. , , Bhutto ، B. نويسنده Department of Veterinary Parasitology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Sindh, Pakistan. , , Memon، A. A. نويسنده Sindh Agriculture University Tandojam, India. , , Shahnaz، I. نويسنده Sindh Agriculture University Tandojam, India. , , Sheeraz، M. نويسنده Sindh Agriculture University Tandojam, India. , , Rao، I. نويسنده Sindh Agriculture University Tandojam, India. ,
Abstract :
In order to optimize a rapid and cost-efficient genomic DNA extraction protocol and to evaluate the effect of extracted DNA stored on various temperatures, 100 fresh tissues of primary sex organs of Kundhi buffalo were collected from local slaughter house. The DNA was extracted using commercial kit, its modified methods and phenol-chloroform method (protocol-4), the fresh and stored DNA was measured through spectrophotometer (Nanodrop 1000). The DNA stored at -20°C, +4°C and at room temperature was degraded in all types of methods of DNA extraction in both types of tissue, the significantly higher degradation was found in the samples when stored at room temperature. In conclusion the both phenol-chloroform and commercial kit yielded a good quality DNA. Genomic DNA stored at -20°C was of good quality, whereas, the storage at room temperature showed degradation more rapidly than other storage conditions.
