Title of article :
A turn on ESIPT probe for rapid and ratiometric fluorogenic detection of homocysteine and cysteine in water with live cell-imaging
Author/Authors :
Goswami، نويسنده , , Shyamaprosad and Manna، نويسنده , , Abhishek and Paul، نويسنده , , Sima and Das، نويسنده , , Avijit K. and Nandi، نويسنده , , Prasanta K. and Maity، نويسنده , , Anup Kumar and Saha، نويسنده , , Partha، نويسنده ,
Issue Information :
هفته نامه با شماره پیاپی سال 2014
Abstract :
5(Benzothiazol-2-yl)-4-hydroxyisophthalaldehyde (BHI), an intense ESIPT containing molecule in mixed media loses its properties due to resonance-assisted H-bond (RAHB) in absolute water. Due to resonance-assisted H-bond the o-aldehyde is more reactive than the other one. With addition of cysteine/homocysteine into this solution the o-aldehyde group gets transformed into thiazolidine/thiazine ring, respectively, and the phenolic proton becomes free enough for transfer to nitrogen of the benzothiazole ring in excited state, that is, the ESIPT of BHI is turned on. Thus we can detect cysteine/homocysteine in water as well as in live cells.
Keywords :
Homocysteine and cysteine sensor , Resonance assisted hydrogen bond , Cell-imaging , ESIPT , Ratiometric sensor
Journal title :
Tetrahedron Letters
Journal title :
Tetrahedron Letters
