Oviposition selection by a rare grass skipper Polites mardon in montane habitats: Advancing ecological understanding to develop conservation strategies

The Grass skipper subfamily (Hesperiinae) includes many at risk species across the globe. Conservation efforts for these skippers are hindered by insufficient information about their basic biology. Mardon skipper (Polites mardon) is declining throughout its range. We surveyed mardon oviposition across nine study meadows in the Gifford Pinchot National Forest of Washington State. We conducted habitat surveys with respect to oviposition (n = 269) and random (n = 270) locations, recording data on over 50 variables. Mardon oviposited on 23 different graminoid species, yet are selective for specific graminoids within meadows. Most frequent ovipositions across meadows occurred on Festuca idahoensis and Poa pratensis (accounting for 112 of 269 total oviposition observations). Discriminant Function Analyses revealed that mardon habitat was too variable to detect oviposition selection across study meadows, yet there was strong selection occurring within meadows (r2 ranging from 0.82 to 0.99). Variables important to within meadow selection were graminoid cover, height, and community; oviposition plant structure (leaf density, height, area); insolation factors (tree abundance and canopy shading); and litter layer factors (cover and depth). With few exceptions the primary variables discriminating between oviposition and random locations were significantly different (p < 0.001). Conservation implications include maintaining native meadow ecosystems with sensitivity to local habitat preferences.