Preliminary studies on cryopreservation of hare (Lepus europaeus Pallas, 1778) semen

In the last decades, a significant decrease in hare population has been observed; for this reason, the aim of the study was to check if hare semen could be preserved in liquid nitrogen, with an extender used for rabbit semen. The results should provide a basis for creating a gene bank of the species. Ten ejaculates (volume above 0.4 ml, percentage of motile spermatozoa above 75%, spermatozoa concentration above 250 × 106 ml), obtained with electroejaculation method from four males, were frozen in an extender of the following composition: Tris (3.028 g), citric acid (1.675 g), glucose (1.25 g), dimethylsulphoxide (DMSO) (4.5%, v/v), egg yolk (17%, v/v) and distilled water to 100.00 ml. The motility of post-thawing spermatozoa was 40.50 ± 7.97%, percentage of spermatozoa with normal acrosomes 76.10 ± 3.69% and percentage of live spermatozoa 35.05 ± 4.21%. Based on the properties of freezing–thawing semen, the hare semen can be successfully preserved in extender used for rabbit semen.