Title of article
Freezing stallion semen with the new Cلceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability
Author/Authors
Morillo Rodrيguez، نويسنده , , A. and Ortega Ferrusola، نويسنده , , C. and Macيas-Garcيa، نويسنده , , B. and Morrell، نويسنده , , J.M. and Rodrيguez Martيnez، نويسنده , , H. and Tapia، نويسنده , , J.A. and Peٌa، نويسنده , , F.J.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2011
Pages
6
From page
78
To page
83
Abstract
Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytometry). Samples frozen in Caceres extender consistently showed the best results in post-thaw motility (increases ranging from 11 to 17%, p < 0.05) and velocity (p < 0.05), membrane integrity (increases ranging from 11 to 14%, p < 0.05) and mitochondrial membrane potential (p < 0.05). It is concluded that this new extender should be included in a freezeability test to determine the best extender for each individual.
Keywords
stallion , Frozen sperm , flow cytometry , CASA
Journal title
Animal Reproduction Science
Serial Year
2011
Journal title
Animal Reproduction Science
Record number
1911542
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