Intrauterine ozone treatment of retained fetal membrane in Simmental cows

The aim of research was to determine influence of intrauterine application of two different ozone preparations on reproductive performance in Simmental cows with retained fetal membranes (RFM). The study was performed on 143 Simmental cows aged 2–8 years and divided in three groups. Group A (n = 46) consisted of cows treated with foam spray ozone applied into the body of the uterus for 5 s using a sterile catheter. Group B (n = 50) consisted of cows treated using six ozone pearls intrauterinely at once, as deeply and hygienically possible. Cows were observed and treated during early puerperium, 24–36 h after parturition. The third group (n = 47) consisted of cows without RFM (control group). To assess the reproductive performance of cows, the interval from calving to first insemination (days not pregnant to first service, DOFS), interval from calving to pregnancy (days not pregnant to pregnancy, DOP), relative pregnancy rate (%), first service conception rate (FSCR, %) and all service conception rate (ASCR, %) were measured. The estimate of hazard ratio for the Groups A and B relative to control group with DOFS were 0.423 (P = 0.0006) and 0.434 (P = 0.0005), and with DOP were 0.701 (P = 0.003) and 0.411 (P = 0.0003), respectively, implying that cows in the control group were not pregnant longer until first insemination and pregnancy. Variables that had an influence on DOFS were postpartum fever (PPF) (HR = 0.458; P = 0.003) and milk yield (HR = 0.999; P < 0.0001) and an influence on DOP were PPF (HR = 0.314; P = 0.001) and milk yield (HR = 0.999; P < 0.0001). Cows with RFM treated with intrauterine Riger spray or Ripromed ovuli O3 have similar or enhanced reproductive performance results compared to the control group of cows demonstrating the effectiveness of therapy with intrauterine ozone products.