Superovulatory response and embryo development in ewes treated with two doses of bovine somatotropin

This study evaluated whether the administration of 50 and 100 mg bovine somatotropin (bST) at the start of synchronization and at the time of natural mating in ewes improves the ovulation rate, embryonic development and pregnancy rate of transferred embryos. Forty-eight donors were assigned to three treatments: the bST-100 treatment (n = 15) received 100 mg bST at the start of synchronization and at natural mating, the bST-50 treatment (n = 15) received 50 mg bST on the same schedule as the previous group, and the control (n = 18) did not receive any bST. Two embryos were transferred to each recipient (n = 121): 35 received embryos from bST-100; 50 received embryos from bST-50, and 36 received embryos from the control. The superovulatory rate, percentage of recovered structures, cleavage rate, percentage of transferable embryos, embryo quality and development and pregnancy rate were analyzed using the GENMOD procedure of SAS. The number of corpora lutea and the cell number were analyzed using the GLM procedure of SAS. The insulin and IGF-1 concentrations were analyzed with ANOVA for repeated measures. The bST application did not affect the superovulatory rate, number of corpora lutea and recovered structures (P > 0.05). The numbers of transferable embryos and embryos reaching the blastocyst were higher (P ≤ 0.01) in the bST-50 (96.4 ± 3.6% and 69.0 ± 7.8%) than the bST-100 (93.0 ± 4.5% and 27.2 ± 38.9%) and control (87.7 ± 5.4% and 50.4 ± 6.4%) groups. The insulin and IGF-1 concentrations were higher (P < 0.05) in the bST-treated groups, but the insulin concentration was higher (P < 0.05) in the bST-100 group than in the bST-50 group. The pregnancy rate was similar (P = 0.21) in ewes receiving embryos from the two treatments [bST-50, (70.0%); bST-100, (62.5%), and control, (56.6%)]. The administration of 50 mg bST at the start of synchronization and at natural mating in superovulated ewes was concluded to enhance the proportion and development of transferable embryos. However, bST did not affect the pregnancy rate of transferred embryos.