Co-digestion of swine manure and corn stover for bioenergy production in MixAlco™ consolidated bioprocessing

MixAlco™ consolidated bioprocessing (CBP) employs a mixed culture of terrestrial microorganisms to anaerobically ferment waste streams (e.g., animal manure, agriculture residues) into mixed carboxylate salts that can be further chemically converted to commodity chemicals (e.g., acetic acid, acetone) and liquid transportation fuels (e.g., ethanol, mixed alcohols, bio-gasoline). For countercurrent fermentations of 60% swine manure/40% lime-treated corn stover at 55 °C, the highest acid productivity [1.8 g/(L·d)] and highest conversion (73%) in this study occurred at an acid concentration of 25.1 and 17.0 g/L, respectively. The continuum particle distribution model (CPDM) predicted the experimental total acid concentrations and conversions around 11.1% and 17.2%, respectively. The CPDM prediction "map" for MixAlco™ CBP indicates that both high conversions (>79%) and high total acid concentrations (>35 g/L) are possible at industrial scale. The present study shows continuous co-digestion of corn stove and swine manure in the MixAlco™ process has the potential to produce annually 9.3 billion gallons of alcohol fuels (e.g., ethanol and mixed alcohols) in the United States.