Author/Authors :
Carrasco، نويسنده , , Mario and Coca، نويسنده , , Raquel and Cruz-Hernandez، نويسنده , , Isabel and Daza، نويسنده , , Silvia and Espina، نويسنده , , Manuela and Garcia-Fernandez، نويسنده , , Emilio and Guerra، نويسنده , , Francisco J. and Leَn، نويسنده , , Rafael and Marchena، نويسنده , , Marيa J. and Pérez، نويسنده , , Inmaculada and Puente، نويسنده , , Manuel and Suلrez، نويسنده , , Esteban and Valencia، نويسنده ,
Abstract :
A kinetic approach to determine the electrostatic potential difference in soft interfaces, when classical methods fail, is presented. The method is based on electrostatic and non-electrostatic separation of the binding constant of one reactant to one of the phases. Here the method is applied to the DNA/water interface. Reliable results are obtained using as probes the reactions [ Ru ( NH 3 ) 5 pz ] 2 + + S 2 O 8 2 - and [Co(NH3)5pz]3+ + [Fe(CN)6]4−.
