Biodegradation kinetics and mechanism of 2,4,6-tribromophenol by Bacillus sp. GZT: A phenomenon of xenobiotic methylation during debromination

A strain Bacillus sp. GZT capable of debrominating and mineralizing 2,4,6-tribromophenol (TBP) was isolated and characterized by morphological observation, biochemical and physiological identification as well as 16S rRNA sequence analysis. Biodegradation kinetics experiments demonstrated that initial TBP concentration had a predominant effect on degradation efficiency. Within 120 h, the highest TBP degradation and debromination efficiencies were up to 93.2% and 89.3%, respectively, under the optimum condition. Ten metabolic intermediates including five brominated compounds, three oxidative products and two cellular metabolites were all identified by gas chromatography–mass spectrometer, and six key intermediates were doubly validated by authentic standards. The proposed biodegradation mechanism inferred that reductive debromination as a major degradation pathway could simultaneously take place at ortho- and para-positions on TBP, while methylated debromination was also found as a minor degradation pathway during this process. Within 148 h degradation, nearly one-third of 3 mg/L TBP could be completely mineralized.