Author/Authors :
Miyake، نويسنده , , T. and Oike، نويسنده , , M. and Yoshino، نويسنده , , S. and Yatagawa، نويسنده , , Y. and Haneda، نويسنده , , K. and Kaji، نويسنده , , H. and Nishizawa، نويسنده , , M.، نويسنده ,
Abstract :
We have studied the coimmobilization of glucose dehydrogenase (GDH) and its cofactor, oxidized nicotinamide adenine dinucleotide (NAD+), on a ketjenblack (KB) electrode as a step toward a biofuel cell anode that works without mediators. A KB electrode was first treated with a sulfuric acid/nitric acid/water mixture to lower the overvoltage for NADH oxidation, and was next chemically modified with NAD+ and GDH. The improved GDH/NAD+/KB electrode is found to oxidize glucose around 0 V vs. Ag/AgCl. A biofuel cell constructed with a bilirubin oxidase-immobilized KB cathode showed a maximum power density of 52 μW/cm2 at 0.3 V.
