Author/Authors :
Gorbenko، نويسنده , , Galyna and Trusova، نويسنده , , Valeriya and Kirilova، نويسنده , , Elena and Kirilov، نويسنده , , Georgiy and Kalnina، نويسنده , , Inta and Vasilev، نويسنده , , Aleksey and Kaloyanova، نويسنده , , Stefka and Deligeorgiev، نويسنده , , Todor، نويسنده ,
Abstract :
The applicability of the novel fluorescent probes, aminoderivative of benzanthrone ABM, squaraine dye SQ-1 and polymethine dye V2 to identification and structural analysis of amyloid fibrils has been evaluated using the lysozyme model system in which fibrillar aggregates have been formed in concentrated ethanol solution. The association constant, binding stoichiometry and molar fluorescence of the bound dye have been determined. ABM was found to surpass classical amyloid marker ThT in the sensitivity to the presence of fibrillar aggregates. Resonance energy transfer measurements involving ABM–SQ-1 and SQ-1–V2 donor–acceptor pairs yielded the limits for fractal-like dimension of lysozyme fibrils.
