Title of article :
Novel magnetic cross-linked enzyme aggregates (magnetic CLEAs) of alpha amylase
Author/Authors :
Talekar، نويسنده , , Sachin and Ghodake، نويسنده , , Vishal and Ghotage، نويسنده , , Trupti and Rathod، نويسنده , , Pavan and Deshmukh، نويسنده , , Priyant and Nadar، نويسنده , , Shamraja and Mulla، نويسنده , , Mosin and Ladole، نويسنده , , Mayur، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Pages :
6
From page :
542
To page :
547
Abstract :
Novel magnetic cross-linked enzyme aggregates of alpha amylase were prepared by chemical cross-linking of enzyme aggregates with amino functionalized magnetite nanoparticles which can be separated from reaction mixture using magnetic field. Of the initially applied alpha amylase activity 100% was recovered in magnetic CLEAs, whereas only 45% was recovered in CLEAs due to the low content of Lys residues in alpha amylase. Scanning electron microscopy analysis showed that CLEAs and magnetic CLEAs were spherical structures. The CLEAs and magnetic CLEAs displayed a shift in optimal pH towards the acidic side, whereas optimal temperature of magnetic CLEAs was improved compared to free enzyme and CLEAs. Although Vmax of enzyme in CLEAs and magnetic CLEAs did not change, substrate affinity of the enzyme increased. The magnetic CLEAs also enhanced the thermal stability and storage stability. Moreover, the magnetic CLEAs retained 100% initial activity even after 6 cycles of reuse.
Keywords :
Amino functionalized magnetite nanoparticles , Alpha amylase , Immobilization , Magnetic cross-linked enzyme aggregates
Journal title :
Bioresource Technology
Serial Year :
2012
Journal title :
Bioresource Technology
Record number :
1930145
Link To Document :
بازگشت