Author/Authors :
Wang، نويسنده , , Qiuyan and Shen، نويسنده , , Linghong and Ye، نويسنده , , Tingting and Cao، نويسنده , , Dan and Chen، نويسنده , , Rong and Pei، نويسنده , , Xiaolin and Xie، نويسنده , , Tian and Li، نويسنده , , Yan and Gong، نويسنده , , Weibo and Yin، نويسنده , , Xiaopu، نويسنده ,
Abstract :
The gene encoding a putative protein from Candida parapsilosis CDC317 (CPE) was cloned and overexpressed in Escherichia coli. The protein was amenable to overexpression in E. coli and constituted 35% of the total cell protein content. The optimal activity was determined at pH 5.5 and 40 °C with the substrate 4-chloro-3-oxobutanoate ethyl ester (COBE). The optical purity of the product was over 99% enantiomeric excess for the (S)-isomer, and the molar conversion yield of the product was 91.1%. The apparent km value for COBE was 0.19 ± 0.01 mM, which is an order of magnitude lower than that of other enzymes in the literature.
