Author/Authors :
Zheng، نويسنده , , Bin and Zettsu، نويسنده , , Nobuyuki and Fukuta، نويسنده , , Megumi and Uenuma، نويسنده , , Mutsunori and Hashimoto، نويسنده , , Tatsuya and Gamo، نويسنده , , Kentaro and Uraoka، نويسنده , , Yukiharu and Yamashita، نويسنده , , Shin-Ichiro and Watanabe، نويسنده , , Heiji، نويسنده ,
Abstract :
We demonstrate a selective nanoscale positioning of targeted Au nanoparticles (NPs) through a bifunctional protein-based encapsulation/delivery system. The newly designed recombinant bifunctional protein, appended with both gold-binding peptide (GBP) and Ti/Si-binding peptide (TBP) at the C- and N-termini efficiently encapsulated 15–20 nm-diameter Au NPs during the pH-controlled reversible reassembly process, and showed the ability of the internalized Au NPs in selective binding to nanometer-scale Ti islands without overshooting. This highly controlled placement of the Au NPs on substrates can be employed to make both large scale devices and point-contact devices.
