Effect of electron donors on anammox coupling with nitrate reduction for removing nitrogen from nitrate and ammonium

Anammox coupling with nitrate reduction under various electron donors was studied using sludge acclimatized to have anammox and denitrification activities. Due to a deficiency in electron donors for NO 3 - reduction, anammox activity in an inorganic medium containing NO 3 - and NH 4 + was lower than that in NO 2 - and NH 4 + . Anammox could use NO 2 - competitively against denitrifiers under a very limited NO 2 - concentration, and additions of swine wastewater or acetate stimulated anammox activity in an inorganic medium containing NO 3 - and NH 4 + with no inhibition effects. However, a high concentration of swine wastewater caused an exponential increase in denitrification activity. The addition of hydrogen and iron stimulated anammox activity in an inorganic medium containing NO 3 - and NH 4 + , but iron showed an inhibitory effect on anammox in a medium containing NO 2 - and NH 4 + . Hydrogen was shown to be advantageous since it did not increase denitrification even when its addition was increased.