Author/Authors :
Li، نويسنده , , Fengcheng and Ren، نويسنده , , Shuangfeng and Zhang، نويسنده , , Wei and Xu، نويسنده , , Zhengdan and Xie، نويسنده , , Guosheng and Chen، نويسنده , , Yan and Tu، نويسنده , , Yuanyuan and Li، نويسنده , , Qing and Zhou، نويسنده , , Shiguang and Li، نويسنده , , Yu and Tu، نويسنده , , Fen and Liu، نويسنده , , Lin and Wang، نويسنده , , Yanting and Jiang، نويسنده , , Jianxiong and Qin، نويسنده , , Jingping and Li، نويسنده , , Shizhon، نويسنده ,
Abstract :
Xylans are the major hemicelluloses in grasses, but their effects on biomass saccharification remain unclear. In this study, we examined the 79 representative Miscanthus accessions that displayed a diverse cell wall composition and varied biomass digestibility. Correlation analysis showed that hemicelluloses level has a strong positive effect on lignocellulose enzymatic digestion after NaOH or H2SO4 pretreatment. Characterization of the monosaccharide compositions in the KOH-extractable and non-KOH-extractable hemicelluloses indicated that arabinose substitution degree of xylan is the key factor that positively affects biomass saccharification. The xylose/arabinose ratio after individual enzyme digestion revealed that the arabinose in xylan is partially associated with cellulose in the amorphous regions, which negatively affects cellulose crystallinity for high biomass digestibility. The results provide insights into the mechanism of lignocellulose enzymatic digestion upon pretreatment, and also suggest a goal for the genetic modification of hemicelluloses towards the bioenergy crop breeding of Miscanthus and grasses.
Keywords :
Biomass digestibility , Miscanthus , xylan , cellulases , Chemical pretreatment
