Author/Authors :
Uchiyama، نويسنده , , Daisuke and Hoshino، نويسنده , , Hajime and Otomo، نويسنده , , Kohei and Kato، نويسنده , , Taro and Onda، نويسنده , , Kenichi and Watanabe، نويسنده , , Akira and Oikawa، نويسنده , , Hiroyuki and Fujiyoshi، نويسنده , , Satoru and Matsushita، نويسنده , , Michio and Nango، نويسنده , , Mamoru and Watanabe، نويسنده , , Natsuko and Sumino، نويسنده , , Ayumi and Dewa، نويسنده , , Takehisa، نويسنده ,
Abstract :
Photoresistance of a pigment-binding membrane protein, light-harvesting 2 (LH2) complex from the photosynthetic bacterium, Rhodopseudomonas acidophila, was investigated by fluorescence of single LH2 complexes at a temperature of 296 K. Before irreversibly stopping fluorescence, a single LH2 complex in phospholipid bilayer of dimyristoylphosphatidylcholine (DMPC) emitted on average 4 times more fluorescence photons than a complex in detergent micelle of octylglucopyranoside (OG). Fluorescence-excitation spectrum of single LH2 complexes taken at 5 K showed that the LH2 complex is structurally less perturbed in DMPC bilayer than in OG micelle, suggesting that structural instability reduces photoresistance of LH2.
