Association of wet disk milling and ozonolysis as pretreatment for enzymatic saccharification of sugarcane bagasse and straw

Ozonolysis was studied separately and in combination with wet disk milling (WDM) for the pretreatment of sugarcane bagasse and straw, with the aim of improving their enzymatic saccharification. The glucose yields for ozonolysis followed by WDM were 89.7% for bagasse and 63.1% for straw, whereas the use of WDM followed by ozonolysis resulted in glucose yields of 81.1% for bagasse and 92.4% for straw, with shorter WDM time. This last procedure allowed a substantial decrease in energy consumption in comparison to the use of WDM alone or of ozonolysis followed by WDM. Higher overall saccharification yields with shorter milling times were observed when ozonolysis was carried out before WDM. This effect might be related to the higher specific surface area. Additionally, a finer morphology was observed by the association of the two treatments in comparison to the sole use of ozonolysis or WDM.