Sweetpotato vines hydrolysate induces glycerol to be an effective substrate for lipid production of Trichosporon fermentans

By co-fermented with sweetpotato vines hydrolysate (SVH), glycerol can be used as an effective substrate for Trichosporon fermentans to produce lipids. Submerged fermentation results showed that T. fermentans exhibited a maximum lipid yield of 7.45 g l−1 with a biomass of 17.95 g l−1 on 10% SVH added glycerol mineral medium (Glycerol MM-10% SVH), which was 4.34-fold higher than that on glycerol mineral medium. Lipids produced on glycerol based media exhibited a significantly different fatty acid composition profile from that produced on sugar based media accompanying by a sharp increase in polyunsaturated fatty acids content. Biochemical behaviors characterization further demonstrated that SVH has a remarkable promoting effect on the biomass formation and glycerol uptake. The extremely high lipid yield on Glycerol MM-10% SVH was mainly attributed to the enhancement of SVH on biomass, although SVH is an excellent nutrient supplier for lipid accumulation due to its low nitrogen availability.