Author/Authors :
Gnedenko، نويسنده , , O.V. and Yablokov، نويسنده , , E.O. and Usanov، نويسنده , , S.A. and Mukha، نويسنده , , D.V. and Sergeev، نويسنده , , G.V. and Bulko، نويسنده , , T.V. and Kuzikov، نويسنده , , A.V. and Moskaleva، نويسنده , , N.E. and Shumyantseva، نويسنده , , V.V. and Ivanov، نويسنده , , A.S. and Archakov، نويسنده , , A.I.، نويسنده ,
Abstract :
The combination of SPR biosensor with electrochemical analysis was used for the study of protein–protein interaction between cytochromes CYP3A4 or 3А5 and cytochromes b5: the microsomal, mitochondrial forms of this protein, and 2 ≪chimeric≫ proteins. Kinetic constants of CYP3A4 and CYP3А5 complex formation with cytochromes b5 were determined by the SPR biosensor. Essential distinction between CYP3A4 and CYP3A5 was observed upon their interactions with mitochondrial cytochrome b5. The electrochemical analysis of CYP3A4, CYP3A5, and cytochromes b5 immobilized on screen printed graphite electrodes modified with membranous matrix revealed that these proteins have very close reduction potentials −0.435 to −0.350 V (vs. Ag/AgCl).
