Title of article
Bioconjugation of concanavalin and CdTe quantum dots and the detection of glucose
Author/Authors
Wang، نويسنده , , Jian-Hao and Li، نويسنده , , Yongqiang and Zhang، نويسنده , , Haili and Wang، نويسنده , , Hai-Qiao and Lin، نويسنده , , Song and Chen، نويسنده , , Jun and Zhao، نويسنده , , Yuan-Di and Luo، نويسنده , , Qing-Ming، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2010
Pages
5
From page
82
To page
86
Abstract
In this paper, water-soluble CdTe/CdS quantum dots (QDs) were synthesized in aqueous solution with glutathione (GSH) as stabilizer. Glutaraldehyde was used as the coupling agent for the conjugation of QDs and concanavalin (con A). Capillary electrophoresis was used to compare the “one-step” method and “two-step” procedure for the conjugation. It was confirmed that the “two-step” approach is more preferable. The achieved bioprobe can be used for the detection of glucose. Under the optimum conditions, the relative fluorescence intensity decreased linearly with the glucose concentration in the range of 0.04–0.2 mM. The detection limit was 0.01 mM. This study provides a new trail of thought for the detection of glucose.
Keywords
glutaraldehyde , Capillary electrophoresis , Quantum dots , Glucose , Concanavalin
Journal title
Colloids and Surfaces A Physicochemical and Engineering Aspects
Serial Year
2010
Journal title
Colloids and Surfaces A Physicochemical and Engineering Aspects
Record number
1939262
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