Prediction of clotting time for milk coagulation by mixtures of proteolytic enzymes

Kinetics of milk coagulation by three proteolytic enzymes, chymosin, bovine pepsin and neutrase, at varying concentrations, were monitored by thrombelastography. Clotting time for milk coagulation by chymosin was linear (r2= 0.997) with the reciprocal of enzyme concentration, whereas the best equation for pepsin (r2 = 0.997) and neutrase (r2 = 0.999) was the one relating the reciprocal of clotting time to enzyme concentration. When the effect of mixtures of enzymes on milk coagulation was studied, the best-fitting regression was that of the reciprocal of clotting time on concentration of enzymes for both chymosin-pepsin (r2 = 0.998) and chymosin-neutrase (r2 = 0.998) mixtures.