Simultaneous determination of total chlorogenic acid, trigonelline and caffeine in green coffee samples by high performance gel filtration chromatography

A method is proposed for the simultaneous determination of total chlorogenic acid (CGA), trigonelline and caffeine in green coffee using high performance gel filtration chromatography. The method presents good linearities (0.9996, 0.9999 and 0.9998) and recoveries (97%, 96% and 96%) with high correlations (0.9158, 0.9715 and 0.9445) for the CGA, trigonelline and caffeine, respectively when compared to reverse phase HPLC techniques. The proposed method appears to be an adequate method for quality control in the coffee industry.