Trimethylamine oxide and derived compoundsʹ changes during frozen storage of hake (Merluccius merluccius)

Changes in total volatile bases (TVB), dimethylamine (DMA), formaldehyde (FA), trimethylamine (TMA) and trimethylamine oxide (TMAO) of whole hake (Merluccius merluccius L.) were evaluated at different temperatures of storage: −5, −12 and −20 °C. Significant changes in TVB, DMA, TMA, TMAO nitrogen and FA were observed at −5 °C. Only TMAO changed significantly at −12 °C and −20 °C. TMAO decrease was not, therefore, followed by DMA and/or TMA production at those temperatures.