Determination of protein in foods: comparison of net protein and crude protein (N × 6.25) values

The purpose of the present study was: (1) to determine net (true) protein (NP) values as sums of amino acid residues and to compare them with crude protein (PA) values; and (2) to calculate the nitrogen-to-NP conversion factors by means of linear regression analyses between the NP values and nitrogen content. The differences between NP and PA values varied between food groups. The NP values in milk and milk products were, on average, 5.5% smaller than the corresponding PA values. The corresponding figures for other foods were: meat and meat products 15.4%, fish and fish products 20.6%, cereals and bakery products 12.2%, vegetables, fruits and berries 13.9% and miscellaneous processed foods 16.1%. In the present study the nitrogen-to-NP conversion factor for milk and milk products was 5.94, meat and meat products 5.17, fish and fish products 4.94, cereals and bakery products 5.40, vegetables, fruits and berries 5.36 and miscellaneous processed foods 5.51; the general conversion factor for all foods was 5.33. The above respective factors were 5.0, 17.3,21.0, 13.6, 14.2, 11.8 and 14.7% smaller than the official factor of 6.25. We conclude that the traditional PA values (N × 6.25) deviate significantly from the NP values. We recommend that protein definitions and methods of determination should be re-evaluated in the appropriate international organizations and updated in line with current knowledge and analytical capabilities for both scientific and other purposes.