Title of article :
Development of a real time PCR assay for detection of allergenic trace amounts of peanut (Arachis hypogaea) in processed foods
Author/Authors :
Lَpez-Calleja، نويسنده , , Inés Marيa and de la Cruz، نويسنده , , Silvia and Pegels، نويسنده , , Nicolette and Gonzلlez، نويسنده , , Isabel and Garcيa، نويسنده , , Teresa and Martيn-Santos، نويسنده , , Rosario، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2013
Pages :
11
From page :
480
To page :
490
Abstract :
Peanut allergic reactions can result from the ingestion of even very small quantities of peanut and represent a severe threat to the health of sensitized individuals. In order to protect the allergic consumer, efficient and reliable methods are required for the detection of allergenic ingredients. For this purpose, we have developed a TaqMan real-time PCR method for specific detection of peanut in commercial food products. The assay uses two genetic makers in the Arah2 (125 bp) and ITS1 (90 bp) gene respectively, and TaqMan probes. The nuclear 18S rRNA gene was employed as a positive amplification control. Results obtained on sensitivity of both Arah2 and ITS primers with mixtures spiked with different concentrations of the target showed detection levels of 10 and 0.1 ppm, respectively. The applicability of the real-time PCR protocol to detect the presence of peanut DNA in commercial food products was determined through analysis of 123 different commercial products with the ITS primers which aimed higher sensitivity than Arah2 primer pair. The performance of the real-time PCR proposed herein allows a highly sensitive detection of peanut DNA in all the different food products, which declared to contain peanut material as well as in others were the presence of peanut or its traces wasnʹt declared in the labeling.
Keywords :
TaqMan real-time PCR , Commercial food products , food allergy , Peanut (Arachis hypogaea)
Journal title :
Food Control
Serial Year :
2013
Journal title :
Food Control
Record number :
1947708
Link To Document :
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