Methyl ketone production from copra oil by Penicillium roqueforti spores

Bioconversion of copra oil by two strains of Penicillium roqueforti spores was studied in the presence or absence of exogenous lipase. Without exogenous lipase action, methyl ketone productivities were weak: 33 ± 1.9 to 61 ± 2.4 μmol g−1 of oil, respectively, for strains 1 and 2. This formation results from the bioconversion of free fatty acids present in copra oil and appears strain-dependent. The major ketone was 2-undecanone, reflecting the high concentration of dodecanoic acid in the substrate. After lipolysis of copra oil by a Candida cylindracea lipase, a large increase of methyl ketone productivities (912 ± 13 and 1935 ± 26 μmol g−1 of oil, respectively) is noticed with 2-heptanone predominating. This observation could result from the selectivity of the lipase used in the bioconversion process, or from the preferential oxidation due to spore specificity. When the reaction time was increased, the amount of methyl ketones decreased and other volatile compounds were formed..