Characterisation of anthocyanins derived from carrot (Daucus carota) cell culture

Two anthocyanin pigments were isolated from cell cultures of Carrot, Nentes scarlet-104 local variety. Chemical hydrolysis, column and paper chromatography, HPLC, proton and 13C NMR and mass spectroscopic studies indicated the presence of cyanidin-3-lathyroside [cyanidin-3-0{β-d-xylopyranosyl (1→2) β-d-galacto pyranoside}] (90%) and cyanidin-3-β-d-glucopyranoside (10%) in the callus cultures, whereas only cyanidin-3-lathyroside (0.05%) was found in the explant carrot. The significant difference was that there was no acylated anthocyanin present as reported in other varieties of carrot.