Title of article :
Species identification of smoked and gravad fish products by sodium dodecylsulphate polyacrylamide gel electrophoresis, urea isoelectric focusing and native isoelectric focusing: a collaborative study
Author/Authors :
Mackie، نويسنده , , I and Craig، نويسنده , , A and Etienne، نويسنده , , M and Jérôme، نويسنده , , M and Fleurence، نويسنده , , J and Jessen، نويسنده , , F and Smelt، نويسنده , , A and Kruijt، نويسنده , , A and Malmheden Yman، نويسنده , , I and Ferm، نويسنده , , M and Martinez، نويسنده , , I and Pérez-Mart??n، نويسنده , , R and Pi?eiro، نويسنده , , C and Rehbein، نويسنده , , H and Kündiger، نويسنده , , R، نويسنده ,
Abstract :
A collaborative study on the use of sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), urea-isoelectric focusing (urea-IEF) and native isoelectric focusing for the identification of species of smoked salmonids, gravad salmonids and smoked eels was carried out by eight laboratories. With SDS-PAGE, minor changes took place in the profiles of the processed salmonid species making it impossible or very difficult to identify closely related species. With urea-IEF, there were fewer changes in the profiles due to processing and the system generally had greater species-discriminating power for the processed salmonids than SDS-PAGE. The profiles of the eel species as obtained on SDS-PAGE or urea-IEF were not affected by smoking. Urea-IEF had greater species-discriminating power than SDS-PAGE for the eel species. Native IEF was useful in providing supplementary identification on species difficult to identify by SDS-PAGE or by urea-IEF in the case of cold smoked products.
