Influence of the hydrophobicity and surface roughness of mangoes and tomatoes on the adhesion of Salmonella enterica serovar Typhimurium and evaluation of cleaning procedures using surfactin

Salmonellosis is associated with the consumption of raw vegetables and fruits such as tomatoes, watermelons, alfalfa sprouts, radishes, carrots, lettuce and parsley. The influence of the fruitsʹ roughness on bacterial adhesion was evaluated as measured using a profilometer. The adhesion of Salmonella enterica serovar Typhimurium to mango and tomato surfaces was also evaluated by measuring of the hydrophobicity of the microorganisms and the fruits surfaces. The bacteria adherent on fruitʹs surface was quantified by plate count and visualize by scanning electron microscopy. In addition, the efficiency of surfactin in removing S. Typhimurium from the fruitsʹ surfaces was analyzed. The average roughness (Ra) of mango (4.54 ± 1.95 μm) was significantly different (p < 0.05) compared to tomato (2.88 ± 2.15 μm). The adhesion of the microorganisms to the fruitsʹ surfaces, as predicted by a determination of the total energy of adhesion (ΔG), was thermodynamically unfavorable. Despite these data, the numbers of bacteria on both fruitsʹ surfaces were similar (p > 0.05), reaching 5.95 ± 0.36 log CFU cm−2 and 5.81 ± 0.39 log CFU cm−2 on mango and tomato, respectively. Therefore, these results suggest that the adhesion observed in this experiment is a multifactorial process. Surfactin removed 94.3% and 92.2% of the S. Typhimurium adhered to the surfaces of the mangoes and tomatoes, respectively. Our research showed that the roughness and hydrophobicity of the fruitsʹ surface did not affect the efficiency of each sanitation treatments on removing of S. Typhimurium. It was observed that the chlorine was more efficient treatment (p < 0.05) for tomato surface. For surface of mangoes, chorine and surfactin were better than water treatment for bacteria control.