Changes in contents of free amino acids and soluble carbohydrates during fruit-body development of Hypsizygus marmoreus

Changes in the contents of free amino acids and soluble carbohydrates in the fruit-body of Hypsizygus marmoreus during its development were investigated. At 25 days (stage A), 27 days (stage B) and 29 days (stage C) after fruiting initiation, fruit-bodies were picked from the cultivation media to determine their free amino acid and soluble carbohydrate contents by using HPLC. Glutamic acid, aspartic acid, asparagine and ornithine were predominant amino acids and their contents, except for ornithine, were higher in the pileus than in the stipe. In contrast, ornithine was much higher in the stipes than in the pilei. In whole fruit-body, the maximal contents of aspartic acid, asparagine and glutamic acid were observed at stage A, whereas ornithine content peaked at stage B. The fruit-bodies contained mannitol and trehalose as major soluble carbohydrates. Their contents were higher in the stipes than in the pilei, and showed a peak at stage B that was at morphological optimum for harvest.