Direct analysis of trans-resveratrol in red wine by high performance liquid chromatography with chemiluminescent detection

A novel high-performance liquid chromatography with chemiluminescence detection has been developed for the determination of trans-resveratrol in red wines based on the enhancement by trans-resveratrol of the chemiluminescence from luminol–potassium ferricyanide system in alkaline medium. Samples were separated on a C8 column using isocratic elution of methanol/water (35:65). Quantification of trans-resveratrol in Chinese red wines was performed without any sample pretreatment. It allows for the determination of trans-resveratrol in the range 0.5–750 μg/L with a detection limit of 0.166 μg/L (S/N=3). The relative standard deviation (RSD) is 1.16% for 7.5 μg/L trans-resveratrol (n=11). trans-Resveratrol was detected in Chinese red wines with the recoveries of 92.2–114.7%. Concentrations ranged from 0.072 to 1.607 mg/L in Chinese red wines.