Title of article :
Production, in continuous enzymatic membrane reactor, of an anti-hypertensive hydrolysate from an industrial alfalfa white protein concentrate exhibiting ACE inhibitory and opioid activities
Author/Authors :
Kapel، نويسنده , , Romain and Chabeau، نويسنده , , Aurelie and Lesage، نويسنده , , Jean and Riviere، نويسنده , , Guillaume and Ravallec-Ple، نويسنده , , Rozenn and Lecouturier، نويسنده , , Didier and Wartelle، نويسنده , , Michel and Guillochon، نويسنده , , Didier and Dhulster، نويسنده , , Pascal، نويسنده ,
Abstract :
A few hydrolysates of food proteins were reported to exert an antihypertensive effect in vivo due to angiotensin I converting enzyme (ACE) inhibitory peptides or opioid peptides. An industrial alfalfa white protein concentrate (AWPC) hydrolysate, produced on a pilot plant scale, strongly inhibited ACE with an IC50 value of 8.8 μg/ml. Furthermore, the AWPC hydrolysate inhibited the spontaneous contraction of rabbit ileum in a dose-dependent and naloxone-blocked manner, indicating the presence of opioid peptides. The AWPC hydrolysate significantly lowered blood pressure of spontaneously hypertensive rats (SHR) more than 6 h after a single oral administration. The maximum reduction of systolic blood pressure (−29.9 ± 2.0 mmHg) was observed 4 h after an oral administration of 0.5 g/kg hydrolysate dosage. The minimal anti-hypertensive effect was observed for 0.1 g/kg dosage. The AWPC hydrolysate may then be particularly suitable as nutraceutical for functional food useful in the prevention of hypertension.
Keywords :
Opioid activity , ACE inhibitory activity , Food protein hydrolysate , Continuous membrane enzymatic reactor , Anti-hypertensive effect , alfalfa
