Quantitative determination and characterisation of the main odourants of Mencيa monovarietal red wines

This study focus on the development of a headspace solid-phase microextraction (HS-SPME) method coupled to gas chromatography–ion trap mass spectrometry (GC–ITMS) to identify and quantify simultaneously 32 characteristic volatile compounds. A mixed level factorial 3 × 23 design was performed to asses the influence of several factors and their interactions (extraction temperature, fibre coating, salt addition and sample volume) on the extraction efficiency. The evaluation of the design showed that temperature and fibre coating were the most significant variables. The method showed good linearity (r2 > 0.990) and repeatability (RSD ⩽ 16%) for all compounds, with detection limits ranging from 0.0024 μg/L for limonene to 238 μg/L for ethyl 3-hydroxybutyrate, in general below their odour threshold limits. Matrix effects were thoroughly studied. The method was after applied to the first aroma characterisation of the monovarietal young red wine Mencía from the Galician (NW Spain) designation of origin “Valdeorras DO”. To achieve the identification of the major would-be impact odourants, the odour activity value (OAV) was also calculated.