Method development for sensitive determination of nisin in food products by micellar electrokinetic chromatography

A rapid and sensitive micellar electrokinetic chromatography (MEKC) method has been developed for the identification and quantification of nisin in food products. Factors such as micelle concentration, pH, and concentration of the buffer were investigated in order to determine the optimum conditions for the analysis. Using optimised conditions of a background electrolyte containing 50 mM sodium phosphate, 80 mM sodium dodecyl sulphate (SDS) at pH 3.75 enabled the successful detection of nisin within 6 min. Limits of detection (LOD) and quantification (LOQ) were in the range of 0.3–0.8 and 1.0–2.8 mg L−1, respectively. The calibration curves were linear for nisin concentration over the range of 2–60 mg L−1. The relative standard deviation (RSD) of the peak area ratios and migration times for method repeatability (n = 3) were found to be lower than 5% and 1%, respectively. The potential of the proposed method to be used for quantitative determination of nisin concentrations in food materials was demonstrated by spiking food samples including dairy products, salad dressings, alcoholic beverages and canned tomatoes with known concentrations of nisin. Quantitative recoveries ranging from 83% to 104% were obtained in the food matrices.