Author/Authors :
Lee، نويسنده , , Jung Han and Lee، نويسنده , , Soo-Jung and Park، نويسنده , , Semin and Kim، نويسنده , , Hae Kyung and Jeong، نويسنده , , Won Young and Choi، نويسنده , , Jin Young and Sung، نويسنده , , Nak-Ju and Lee، نويسنده , , Won Sup and Lim، نويسنده , , Chae-Shin and Kim، نويسنده , , Gon-Sup and Shin، نويسنده , , Sung Chul، نويسنده ,
Abstract :
Orostachys japonicus cultivated in the Republic of Korea was analysed for flavonoid content via HPLC coupled to MS/MS. Amongst 16 compounds that were characterised, eight flavonoids and one alkaloid were characterised for the first time: two procyanidin dimer gallate isomers (1 and 2), epigallochatechin-3-gallate (3), two procyanidin dimer digallate isomers (4 and 9), quercetin 3-O-rhamnosyl-7-O-glucoside (6), myricetin 3-O-glucoside (10), kaempferol (16) and N1,N5,N10-tri-p-(E,E,E)-coumaroylspermidine (15). The identified compounds were quantified by HPLC–UV/DAD. The antioxidant activity of the O. japonicus flavonoids was determined via 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) radical cation (ABTS•+) and nitric oxide radical (NO•) scavenging assays.
Keywords :
Flavonoids , Orostachys japonicus , Antioxidant effect , HPLC–MS/MS , Quantification
