Determination of melamine by flow injection analysis based on chemiluminescence system

In this paper, based upon the phenomenon that melamine can obviously enhance the CL signal of the luminol–H2O2 system in basic medium, a simple, rapid and sensitive flow injection chemiluminescence (FI-CL) method for the determination of melamine has been developed. Under the optimum conditions, the linear range for the determination of melamine was 0.2–80 μg mL−1 with a detection limit of 0.12 μg mL−1 calculated as proposed by IUPAC and a relative standard deviation of 3.26% for 11 solutions of 10 μg mL−1 melamine on the same day. The proposed method was satisfactorily applied to determine melamine in milk-based products and satisfactory results were obtained without interferences from the sample matrix. Moreover, one assay produce takes only 25 s and the minimum sampling rate is about 120 samples h−1, which indicated that the FI-CL method was suitable for high throughput and real-time melamine analysis.