Author/Authors :
Fernandez-Lorente، نويسنده , , Gloria and Bolivar، نويسنده , , Juan Manuel and Rocha-Martin، نويسنده , , Javier and Curiel، نويسنده , , José A. and Muٌoz-Guerra، نويسنده , , Rosario and de las Rivas، نويسنده , , Blanca and Carrascosa، نويسنده , , Alfonso V. and Guisan، نويسنده , , Jose M.، نويسنده ,
Abstract :
Immobilised derivatives of tannase from Lactobacillus plantarum were able to catalyse the transesterification of tannic acid by using moderate concentrations of 1-propanol in aqueous media. Transesterification of tannic acid was very similar to transesterification of methyl gallate. The synthetic yield depended on the pH and concentration of 1-propanol, although it did not vary much when using 30% or 50% 1-propanol. Synthetic yields of 45% were obtained with 30% of 1-propanol at pH 5.0. The product was chromatographically pure, and the reaction by-product was 55% pure gallic acid. On the other hand, immobilised tannase was fairly stable under optimal reaction conditions.
