Determination of anti-tubercular agent in mango ginger (Curcuma amada Roxb.) by reverse phase HPLC-PDA-MS

Mango ginger (Curcuma amada) is a spice of high usage in pickles, sauce, culinary formulations and traditional/folk systems of medicine for therapeutic actions in Asian countries. After establishing scheme for isolation and characterisation of labdane diterpene dialdehyde [labda-8(17), 12-diene-15, 16-dial]-an anti-tubercular agent, we developed a new validated HPLC-PDA method for its quantification in C. amada. Chromatography was performed with reverse phase column in isocratic condition at a flow rate of 1.0 mL/min using mobile phase of acidified water and acetonitrile. Specificity of determination was achieved with UV (190–400 nm) and mass spectrum. Good linearity was obtained with correlation coefficients >0.99. In the analytical range the precision and accuracy (RSD%) values were ⩽2%. Present validated method allowed both the identification and determination of labda-8(17), 12-diene-15, 16-dial, in C. amada rhizome. The method was applied to screen the labdane diterpene dialdehyde in the samples of different geographic locations.