Preparation of sesame peptide and evaluation of antibacterial activity on typical pathogens

The antibacterial activity of sesame peptides on common pathogen was evaluated. The preparation of sesame protein hydrolysate was carried out in an enzymatic membrane reactor followed by fractionation through several ultrafiltration stages to obtain desired peptide fractions and evaluation of their antibacterial activity using two pathogens namely, Pseudomonas aeruginosa and Bacillus subtilis. Potential sesame peptides were characterized by mass spectroscopy (TOF-MS) followed by determination of their corresponding amino acid compositions. Sesame peptide fraction of molecular mass less than 1 kDa exhibited significant inhibition against growth of P. aeruginosa as compared to B. subtilis. Thus, these findings confirm the bacteriostatic effect of sesame peptide on growth of pathogens which can be exploited in bacteriostat composition.