Simultaneous multiresidue determination of mycotoxins in cereal samples by polyvinylidene fluoride membrane based dot immunoassay

The development of polyvinylidene fluoride (PVDF) membrane-based dot immunoassay for rapid and simultaneous detection of multi-mycotoxins in cereal samples is described. To facilitate the simultaneous identification of multiple mycotoxins in a single test, membrane reaction zones were constructed using PVDF membrane, rubber fences and scotch tape. The cut-off level for this method, assessed visually, were 20, 60, 1000, 20, and 250 μg kg−1 for Aflatoxin B1, zearalenone, deoxynivalenol, ochratoxin A, and fumonisins B1, respectively, and the final results can be obtained within 10 min. This simultaneous method was designed to be simple, with no time-consuming cleanup procedure, and good accuracy and reproducibility were obtained in recovery experiments. These results suggest that this method could be a useful on-site screening tool for the rapid detection of multiple mycotoxins in cereal samples without special instrumentation.