Author/Authors :
Heidari Keshel، Saeed نويسنده Proteomics Research Center, Faculty of Paramedical Sciences , , Soleimani، Bita نويسنده Department of Chemistry, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran , , Ebrahimi، Maryam نويسنده Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. , , Ashrafi Hafez، Asghar نويسنده Safety promotion and injury prevention research center, Shahid Beheshti University of medical sciences, Tehran , , Fallahakbarpour، Razieh نويسنده Department of Biomaterials Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran , , Khaledian، Mohammad Ali نويسنده , , Yousefi، Fatemeh نويسنده Babol University of Medical Sciences, Babol, Iran , , Rostampour Kakroudi، Maryam نويسنده Department of Biomaterials Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran. , , Fathi Arateh، Forough نويسنده Department of Biomaterials Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran. ,
Abstract :
Thermo-sensitive polymers were prepared by graft copolymerization of gelatin with N-Isopropylacrylamide via gamma radiation. Characterization of polymers such as DSC analysis, swelling in different ratios and cell assays were investigated. DSC and solubility analysis showed gelatin and N-Isopropylacrylamide monomers were grafted via gamma radiation successfully. Results show swelling of samples increased as gelatin increased. Swelling ratio and curves results administrated hyd-ro-philicity / hydrophobicity of hydrogel that this property is due to presence of N-Isopropylacrylamide in different temperatures. The polymer was tested for harvesting epithelial cells after carrying out cell culture at 37 °C and incubating the confluent cells at 10°C for spontaneous detachment of cell sheet from polymer surface without enzyme treatment. These unique properties of the hydrogel would make it a promising support for drug delivery systems and tissue regeneration.
