Author/Authors :
Nishio، نويسنده , , Kosuke and Masaike، نويسنده , , Yuka and Ikeda، نويسنده , , Morihito and Narimatsu، نويسنده , , Hiroki and Gokon، نويسنده , , Nobuyuki and Tsubouchi، نويسنده , , Shingo and Hatakeyama، نويسنده , , Mamoru and Sakamoto، نويسنده , , Satoshi and Hanyu، نويسنده , , Naohiro and Sandhu، نويسنده , , Adarsh and Kawaguchi، نويسنده , , Haruma and Abe، نويسنده , , Masanori and Handa، نويسنده , , Hiroshi، نويسنده ,
Abstract :
We developed novel magnetic nano-carriers around 180 nm in diameter for affinity purification. Prepared magnetic nano-carriers possessed uniform core/shell/shell nano-structure composed of 40 nm magnetite particles/poly(styrene-co-glycidyl methacrylate (GMA))/polyGMA, which was constructed by admicellar polymerization. By utilizing relatively large 40 nm magnetite particles with large magnetization, the magnetic nano-carriers could show good response to permanent magnet. Thanks to uniform polymer shell with high physical/chemical stability, the magnetic nano-carriers could disperse in a wide range of organic solvent without disruption of core/shell structure and could immobilize various kinds of drugs. We examined affinity purification using our prepared magnetic nano-carriers with anti-cancer agent methotrexate (MTX) as ligand. Our magnetic nano-carriers showed higher performance compared to commercially available magnetic beads in terms of purification efficiency of target including extent of non-specific binding protein.
Keywords :
magnetite , Polymerization , Magnetic carrier , drug target , Affinity purification
