Voltammetric determination of hypoxanthine based on the enhancement effect of mesoporous TiO2-modified electrode

A mesoporous TiO2 was synthesized according to the reported method, and then used to modify the carbon paste electrode (CPE). The electrochemical behavior of hypoxanthine was investigated with great detail. Compared with the unmodified CPE, the mesoporous TiO2-modified CPE greatly enhances the oxidation signal of hypoxanthine. Due to huge surface area, well-defined and special mesopores, the mesoporous TiO2-modified CPE shows considerable enhancement effect toward hypoxanthine. Based on this, a sensitive, rapid and convenient electrochemical method was developed for the determination of hypoxanthine. The linear range is over the range from 2.0 × 10−7 to 5.0 × 10−5 mol L−1, and the limit of detection is estimated to be 5.0 × 10−8 mol L−1. The relative standard deviation (RSD) for 10 mesoporous TiO2-modified CPEs is 5.7%. Finally, this sensing method was successfully used to determine hypoxanthine in human blood serum samples.